Immunoanalytical methods for ochratoxin A monitoring in wine and must based on innovative immunoreagents

Abstract Immunochemical methods are highly deployed in analytical laboratories worldwide for monitoring the incidence of mycotoxins food chain. Nevertheless, most conventional immunoassays ochratoxin A (OTA), including commercial kits, show limitations to robustly determine this mycotoxin grape-derived products below regulated levels (2 ng/mL). Herein, two rapid tests sensitive OTA determination wine and must were developed capitalizing on a collection bioconjugates from innovative synthetic haptens monoclonal antibodies with subnanomolar affinity. The ELISA (LOD = 8 pg/mL) showed excellent performance recovery studies, it was applied survey wines musts contamination. Concerning LFIA, validation according Commission Regulation 519/2014 that samples exceeding 2 ng/mL properly scored as uncompliant. More importantly, illegal provided complete inhibition test signal, making an easy-to-use, rapid, convenient screening method in-house control wineries.